Abstract
SONICATION ASSISTED AGROBACTERIUM MEDIATED TRANSFORMATION
(SAAT): AN ALTERNATIVE METHOD FOR COTTON TRANSFORMATION
Pak. J. Bot., 39(1):
223-230, 2007.
SYED SARFRAZ HUSSAIN*, TAYYAB HUSNAIN1 AND S. RIAZUDDIN1
Department of Biosciences, COMSATS Institute of Information Technology,
Plot # 30, H-8/1, Islamabad, Pakistan
We report here a new procedure for cotton transformation based on cavitations
caused by sonication which results in thousands of micro wounds on and
below the surface of plant tissue and allow Agrobacterium to
travel deeper and completely throughout the tissue. This wounding fashion
increases the probability of infecting plant cells lying deeper in tissue.
Many parameters were optimized for the enhancement of GUS transient
expression in cotton using mature embryos as explant. GUS was first
detected 24h following incubation of the explants and by 48h, GUS expression
was very intense which served as a useful indicator of successful transformation
of the cotton explant following sonication assisted Agrobacterium
mediated transformation (SAAT) procedure. The study also showed
the competitive advantage of this procedure over other transformation
procedures being routinely used.
1 National Centre of Excellence in Molecular Biology, University of
the Punjab,
87-West Canal Bank Road Thokar Niaz Baig Lahore-53700 Pakistan
* Corresponding E. Mail: syedsarfraz_2000@yahoo.com
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