AbstractIN VITRO CALLUS INDUCTION, ITS PROLIFERATION AND REGENERATION IN SEED EXPLANTS OF WHEAT (TRITICUM AESTIVUM L.) VAR.LU-26S
Pak. J. Bot., 35(2): 209-217, 2003.MOHAMMAD IHSAN SHAH, MUSSARAT JABEEN AND IHSAN ILAHI
Department of Botany, University of Peshawar, Peshawar, Pakistan.
The present study was undertaken to evaluate the most suitable concentration of growth regulators i.e., 2,4-D, IAA, BAP and Kin for callus induction, its proliferation and regeneration in seed explants of wheat (Triticum aestivum L) Lu-26S. Good callus formation was obtained on MS medium containing 3.5-mg/l 2,4-D. A medium containing 0.5 mg/l BAP in combination with 2.0 mg/l 2,4-D and 4.0mg/l BAP alone also gave good response and a good callus proliferation was obtained. Excellent regeneration was observed in a medium with 4.0 mg/l BAP used alone and 2.0mg/l BAP in combination with 1.0 mg/l IAA.
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